Author:
Chen Hsin-Hsien,Ho Chia-Shin,Hsu Ming-Hung,Lin Yu-Chen,Chang Jui-Feng,Yang Shieh-Yueh
Abstract
<b><i>Background:</i></b> For assays using immunomagnetic reduction, a reagent composed of antibody-functionalized magnetic nanoparticles is dispersed in phosphate-buffered saline solution. The real-time signals of alternating-current (ac) magnetic susceptibility, χ<sub>ac</sub>, of the reagent are subsequently recorded after mixing the reagent with a biofluid sample. After mixing the reagent and sample, the reduction in χ<sub>ac</sub> of the mixture is calculated and used to quantify the concentration of the target biomarker in the sample. The reduction does not occur immediately but rather occurs at some time after mixing. This observation implies that the time elapsed before recording the real-time signals of χ<sub>ac</sub> of a reagent-sample mixture needs to be investigated to ensure that the signals are fully recorded. In this work, the effect of time to detection on the measured concentrations of proteins in human plasma after mixing the reagent and sample is examined. <b><i>Methods:</i></b> The proteins analyzed are related to Alzheimer’s disease: amyloid β 1–40, amyloid β 1–42, and Tau protein. The investigated times to detection after the mixing the reagent and sample are 0, 20, 30, 40, and 120 min. <b><i>Results:</i></b> The results show that the recording of real-time signals of χ<sub>ac</sub> should be conducted within 20 min after mixing the reagent and sample.
Subject
Psychiatry and Mental health,Cognitive Neuroscience
Cited by
1 articles.
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