Pathological Analysis of Early Transplant Glomerulopathy in Renal Allografts Using Low-Vacuum Scanning Electron Microscopy-Reference-Cited by-同舟云学术

Pathological Analysis of Early Transplant Glomerulopathy in Renal Allografts Using Low-Vacuum Scanning Electron Microscopy

Published:2020 Issue:1 Volume:144 Page:71-78
ISSN:1660-8151
Container-title:Nephron
language:en
Short-container-title:Nephron

Author:

Onishi Hiroka,Oguchi Hideyo,Shinoda Kazunobu,Mikami Tetuo,Arai Taichi,Yamaguchi Yutaka,Sakai Ken

Abstract

Aim: Low-vacuum scanning electron microscopy (LVSEM) has been reported to aid in diagnosis of renal biopsy. This study evaluated early transplant glomerulopathy in kidney transplant recipients using LVSEM. Methods: We selected 4 biopsies of cg0, 5 biopsies of cg1a, 5 biopsies of cg1b, and 4 biopsies of cg2 lesions that had been evaluated by light microscopy (LM) and transmission electron microscopy from recipients with acute/active or chronic, active antibody-mediated rejection (AABMR or CAABMR). Renal allograft paraffin sections (1 µm thickness) were stained with periodic acid-methenamine silver and observed using LVSEM. The cg score was based on the Banff classification. The parameter “percentage of duplicated capillary number” was calculated as follows: in 1 glomerulus with glomerular basement membrane (GBM) duplication, the total duplicated capillary number/the total number of capillaries ×100. Results: In all 4 biopsy specimens with AABMR showing cg0, LVSEM revealed GBM duplication not identified by LM. The average percentage of duplicated capillary number per glomerulus with GBM duplication was higher when observed by LVSEM than when observed by LM in all cg1b and cg2 biopsy specimens. Conclusion: LVSEM revealed early GBM duplication in AABMR. Early GBM duplication might progress in the very early phase of AABMR. GBM duplication was more frequently detected by LVSEM than by LM in biopsy specimens with early chronic, active antibody mediated rejection. Thus, LVSEM may be useful in diagnosis of early transplant glomerulopathy.

Publisher

S. Karger AG

Reference11 articles.

1. Gloor JM, Sethi S, Stegall MD, Park WD, Moore SB, DeGoey S, et al. Transplant glomerulopathy: subclinical incidence and association with alloantibody. Am J Transplant. 2007;7(9):2124–32.

2. Haas M, Mirocha J. Early ultrastructural changes in renal allografts: correlation with antibody-mediated rejection and transplant glomerulopathy. Am J Transplant. 2011;11(10):2123–31.

3. Haas M, Sis B, Racusen LC, Solez K, Glotz D, Colvin RB, et al. Banff 2013 meeting report: inclusion of c4d-negative antibody-mediated rejection and antibody-associated arterial lesions. Am J Transplant. 2014;14(2):272–83.

4. Inaga S, Kato M, Hirashima S, Munemura C, Okada S, Kameie T, et al. Rapid three-dimensional analysis of renal biopsy sections by low vacuum scanning electron microscopy. Arch Histol Cytol. 2010;73(3):113–25.

5. Okada S, Inaga S, Kawaba Y, Hanada T, Hayashi A, Nakane H, et al. A novel approach to the histological diagnosis of pediatric nephrotic syndrome by low vacuum scanning electron microscopy. Biomed Res. 2014;35(4):227–36.

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Recent advances in electron microscopy for the diagnosis and research of glomerular diseases;Kidney Research and Clinical Practice;2023-03-31

2. A novel technique of STEM observation of TEM section using LVSEM and application of pathological diagnosis of renal biopsy;Microscopy;2022-10-21