Affiliation:
1. Department of Internal and Preventive Veterinary Medicine. College of Veterinary Medicine. AL-Qasim Green University, Babylon, Iraq
2. Al-Qasim Agriculture Division, Babylon Agriculture Directorate
Abstract
Equine infectious anemia (EIA) is a viral infection that affects Equidae as a persistent infection. Infected horses usually develop a systemic disease with mild
clinical signs such as irregular fever, weight loss, anemia, and weakness. The
study was the first molecular record of the EIA viruses in horses in Iraq. In total,
72 blood samples (30 horses and 42 donkeys) were selected with history and
signs of anemia. The study was conducted in central Iraq (Babylon, Karbala, and
Al-Dawinih provinces) from 2015 to 2017. Then, primer design and molecular
identification of the gag gene of the EIA virus were performed. The results
showed that EIA virus infection rates in horses and donkeys in Iraq's middle regions were 16.66% and 0%, respectively. The DNA sequences of two positive
EIA virus samples revealed that isolates have 271 nucleotides under accession
numbers MN861108.1 and MN861109.1, respectively. The phylogenetic analysis
revealed that Iraqi isolates were clustered in different clades and revealed 83.1–
100% identity with EIAV world isolates in the NCBI. This study was the first
molecular record of EIA viruses in Iraq. The study indicated that the gag region
was an essential target for EIAV detection.
Keywords: PCR, Gag, Gene, EIA, Iraq
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
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