Antimicrobial effect of Morenga oleifera seeds extract on bacterial isolates from wounds and burns

Abstract

The present study was carried out to investigate the antibacterial effect of Moringa oleifera seed extract (MOSE) against bacterial isolates (A. baumannii, K. pneumoniae and S. aureus). Thirty-eight bacterial isolates were collected from wounds and burns of hospitalized patients from Baghdad city: A. baumannii (13), K. pneumoniae (7) and S. aureus (18). All isolates were identified based on phenotypic, microscopic, biochemical, API 20 diagnostic system. The preparation of (MOSE) by the ethanolic extraction method showed no antimicrobial activity against the bacterial isolates. Thus, the screening was done using only the Moringa oleifera seeds extract from the aqueous extraction method. The results revealed that (MOSE) in the disc diffusion method has some levels of inhibition only against S. aureus isolates with inhibition zones (10 mm, 9 mm, Weak inhibition) for concentrations (1000, 500 and 250 mg/ml), respectively. Meanwhile, in the Agar-well diffusion method the antibacterial activity of (MOSE) against bacterial isolates with an inhibition zone at 1000 mg (14-15 mm, 12-15 mm, 18-15 mm) for A. baumannii, K. pneumoniae and S. aureus respectively and at 500 mg/ml (11 mm, 14-12 mm, 14-13 mm) for A. baumannii, K. pneumoniae and S. aureus respectively. Keywords: Moringa oleifera; K. pneumoniae; S. aureus; A. baumannii