The availability of the vitamin D among children with allergic disease caused by the polymorphic gene VDR

Abstract

Objective: The objective is to analyze the availability of vitamin D in children with allergic diseases (AD) in polymorphism of the vitamin D receptor (VDR) gene.Methods: The main group included 130 children with allergic diseases aged from 1.5 to 16 y.o. The control group included 41 apparently healthy children aged from 1 to 10 y.o. The analysis of polymorphic markers FokI (rs2228570), BsmI (rs 1544410) and TaqI (rs 731236) of gene VDR was carried out by polymerase chain reaction method in realtime mode using detecting amplifier DT-96 and DNA-diagnostics sets. The assay of metabolite 25(OH) D (25-Hydroxyvitamin D2 and D3) was carried out by an immunoenzyme method.Results: Children with AD demonstrated a significantly increased degree of incidence of A-allele in the site of BsmI gene VDR and carriage of homozygous (A/A) and heterozygous (G/A) of its genotypes. The statistically significant decrease of 25 (OH) concentration was established in heterozygous variant A/G and in homozygous variant G/G of FokI site of gene VDR.Conclusions: The findings lay the groundwork for development of individual approach to prevent vitamin D deficiency in children with AD.