Structural And Functional Annotation Of Rv1514c Gene Of Mycobacterium Tuberculosis H37Rv As Glycosyl Transferases

Abstract

We need an emergent cure against tuberculosis after seeing its capability of scattering all around the world. The causative pathogen Mycobacterium tuberculosis (M. tuberculosis) persistently gets accomplishment in spreading the repulsiveness of overall expanding death rate; thusly create an emergent requirement for curing people to interact with this pathogen. There might be numerous points of view of this bacterium to take a shot for focusing on the imperative components for its development and survival, one of those systems is recorded in the article underneath. Glycosylation is the universal and one of the most important phenomenon’s for modification. This gene is a suitable one for studying exact functioning of glycosyl transferases enzymes as these are the non essential ones and require for growth of this bacterium. The target of the manuscript is to accentuate the In silico highlights of the Rv1514c gene of M. tuberculosis which prove being an essential role like transferases activity, transferring glycosyl group’s action. For the study of Rv1514c gene, it comprises 789 bp long which codes for nearly approx 29kDa protein. In String database server for functional partner prediction demonstrates it interact with an fcl (EpiA), Rv1508A, gmdA, Rv1516c, Rv3264c, Rv1515c, Rv3032, Pima, Rv2188c and glgA. Rv1514c modelled by using I-TASSER server and for model evaluation we using ProSA, Rampage, Verify 3D and ERRAT which outcome score are satisfactory. In Rv1514c binding sites prediction done by COACH which confirmed this gene might be bounded with UDP (Uridine-Diphosphate) and it could be a Transferases activity protein which possibly, transferring glycosyl groups action. Assist exploratory investigations of this gene might be valuable in giving physiological and biological vitality as a remedial. Keywords: Mycobacterium tuberculosis; Rv1514c; Glycosylation; Macrophages; Transferases