Hepatoprotective Activity of Amomum Subulatum (Roxb.) Seeds against ParacetamolInduced Liver Damage in Rats

Abstract

Hepatoprotective activity of methanolic extract of Amomum subulatum seeds (MEAS) was evaluated against paracetamol (3 g/kg, p.o. for 3 days) induced liver damage in rats. The degree of protection was measured using serum biomarkers like Alanine and Aspartate aminotransferase, alkaline phosphatase, total and direct bilirubin, lactate dehydrogenase, gamma glutamyl transferase and total protein; oxidative stress parameters such as malondialdehyde, glutathione, super oxide dismutase and catalase along with histopathology. Significant higher levels of above serum biomarkers, whereas lower level of total protein in paracetamol control as compared to normal control was observed. Treatments with MEAS and silymarin caused significant reversal in above biomarkers which was altered by paracetamol. Similarly, significant increase in MDA while decrease in GSH level, SOD and CAT activities in paracetamol control as compared to normal control. Treatment with MEAS and silymarin brings these stress parameters changes significantly towards normal level as compared to paracetamol control. Histopathological changes were in the same direction supports finding of above biomarkers and stress parameters. These results suggest that MEAS may have potential therapeutic value to reduce the severity of paracetamol induced liver damage, probably by its antioxidative effect on liver by eliminating the deleterious effects of toxic metabolites of paracetamol.