Characterization and evaluation of the hepatoprotective activity of α-mangostin isolate in diabetic rats

Abstract

Introduction: Isolation of α-mangostin (α-MG) from the rind extract of Garcinia mangostana L. showed antioxidant, anticancer, antidiabetic, and cytotoxic activities. The effectivity of a-mangosteen isolate compounds on hepatoprotective bioactivity in diabetic conditions has not been found. This study aimed to characterize α-MG isolate and evaluate its hepatoprotective activity in diabetic rats. Methods: This research was a post-test-only control group design. This study used 36 rats which were divided into 6 groups, namely positive control group, negative control group, standard drug group, and treatment group with α-MG dose of 10 mg/kg body weight, 30 mg/kg body weight, and 50 mg/kg body weight. Hepatoprotective evaluation parameters examined included levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin and liver histopathology observations. Test the effect on the study using the One-Way ANOVA test. Results: The results showed that a-mangosteen compound has a significant effect on levels of AST (p-value<0.05), ALT (p-value<0.05), ALP (p-value<0.05), and total bilirubin (p-value<0, 05), but there were no necrotic cells on the histopathological observation of the liver. Conclusion: α-mangostin isolate from Garcinia mangostana L. has the potential as hepatoprotective in diabetic rats.