Carbon Quantum Dots Coupled Au Nanoparticle as Fluorescence-Based DNA Biosensors for Dengue Virus Detection

Author:

Monday Yakubu Newman,Abdullah Jaafar,Yusof Nor Azah,Abdul Rashid Suraya,Shueb Rafidah Hanim,Sidek Hamidah

Abstract

This study introduces a novel DNA biosensor probe comprising carbon quantum dots (CQDs) derived from palm kernel shell biomass and gold nanoparticles (AuNPs) synthesized via the citrate reduction method. The CQDs were doped with ethylenediamine using a hydrothermal process employing a one-pot synthesis method in an autoclave batch reactor. The resulting CQDs exhibited exceptional photoluminescent (PL) properties, with an excitation wavelength of 360 nm and an emission wavelength of 430 nm. Transmission electron microscope (TEM) images showed the average particle sizes of the CQDs and AuNPs to be 2 nm and 15 nm, respectively. Carboxylic acid-modified CQDs were coupled to amine-modified ssDNA (PA) to construct the biosensor through the amine coupling technique. The AuNPs were modified through thiol coupling with Rhodamine B, L-cysteine, and thiol-modified ssDNA (PT). Both PA and PT probes were designed to complement the DEN-3 virus oligonucleotide. CQDs acted as fluorophores and energy donors in the biosensor, while the AuNPs functioned as nanoquenchers of fluorophores and energy acceptors. The resulting probe pair, CQDs-PA, and AuNPs-PT demonstrated remarkable Förster resonance energy transfer (FRET) and exhibited fluorescence turn-on upon titration with DEN-3. The biosensor displayed excellent sensitivity with a logarithmic calibration equation of 5.22LogC + 20.79 (R2 = 0.979), covering a linear range of 0.001 nM to 100 nM. The limit of detection (LOD) was determined to be 1.57 ± 0.71 nM. This innovative DNA biosensor, incorporating CQDs and AuNPs, holds promising potential for sensitive and specific detection of the DEN-3 virus.

Publisher

Penerbit Universiti Kebangsaan Malaysia (UKM Press)

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3