Synthesis and Characterization of Dose-Dependent Antioxidants and Antimicrobial Activity of Phloretin Loaded PLGA Nanoparticles

Abstract

This aim of the study combines and differentiates Phloretin-encapsulated PLGA (poly (dl-lactide-coglycolide) nanoparticles and assesses their antioxidant and antibacterial properties. Phloretin is a possible active ingredient with a wide range of therapeutic effects. The process of emulsification was used to make nanoparticles. Physicochemical properties were measured by Particle size, polydispersity index, and zeta-potential investigated by dynamic light scattering (DLS), X-ray diffraction, FTIR, TEM, and AFM. FTIR results proven that phloretin formulated PLGA NPs exhibited functional groups. The Zeta potential size analyzer results of the optimal NPs size were 98.7 nm. The crystallinity nature peaks related to Phloretin-PLGA NPs were confirmed by XRD. Antioxidant properties of free and encapsulated nanoparticles were determined by DP, method. Relatively small polymeric nanoparticles were obtained containing phloretin. DPPH radical scavenging activity of the nanoparticles was found to be 71% at a 1 mg/ml concentration. It also had antibacterial properties when examined using the good diffusion approach against Negative species. In conclusion, our findings show that encapsulated PLGA phloretin nanoparticles with continuous release properties retain antioxidant and antimicrobial activity, implying therapeutic potential.