Effect of systemic ozone use on oxidative modification of lipids and proteins in the colon in experimental colitis

Abstract

Introduction. The processes of free radical oxidation play a significant role in the pathogenesis of inflammatory bowel diseases. The aim of the work was to study the clinical status, the content of lipid peroxidation products, oxidative modification of proteins in the lesion of the colon in oxazole-induced colitis (OIC) under conditions of intraperitoneal application of ozone. Materials and methods. Wistar rats were modeled for OIC using oxazolone solution. Ozone-oxygen mixture (OX) was injected intraperitoneally once a day for six days. The clinic was assessed by disease activity index (DAI), the content of products of lipid peroxidation (LPO) and oxidative modification of proteins (OMB) was determined in colonic homogenate. Results. Under OIK DAI increases, the level of primary and secondary products in the heptane phase increases in the colonic homogenate; the level of secondary products and end products increased in the isopropanol phase. Under conditions of intraperitoneal application of ozone, DAI decreased, the level of isopropanol-soluble primary, secondary, final LPO products increased in colon homogenate on the 2nd day, the level of heptane- and isopropanol-soluble primary, secondary, final LPO products decreased on the 6th day, early and late LPO products decreased on the 4th, 6th day. We found a moderate and significant relationship on the Cheddock scale between DAI and the content of LPO and OMB products in the colonic homogenate mainly on day 6 of OIC under conditions of intraperitoneal application of ozone. Discussion. The increased content of LPO and OMB products in the lesion of the colon after the use of ozone is probably due to its mediated action (through the activation of ROS) and its ability to act as an oxidant of lipids and proteins of the cells of the mucosa of the colon. Conclusions. The positive effects of intraperitoneal application of ozone in OIC are the basis for further research in studying the mechanism of the protective effect of ozone with the possibility of further application in clinical conditions in inflammatory bowel diseases.