Affiliation:
1. Division of Human Nutrition, University of British Columbia, Vancouver, Canada.
Abstract
Human cholesterogenesis is measurable as the rate of incorporation of deuterium derived from deuterium oxide (D2O) within the body water pool into plasma or erythrocyte cholesterol pools. Oral D2O equilibrates across body water, thus enabling extracellular sampling of pools (such as urine) to serve as accurate indicators of intracellular deuterium enrichments at the point of synthesis. Required doses of D2O fall below the threshold associated with negative side effects. Deuterium/carbon incorporation ratios into cholesterol during biosynthesis have been established that are applicable in humans. Models using unconstrained and constrained curve fitting permit improved flexibility in interpretation of deuterium-uptake kinetics. However, sample-size restrictions presently limit the ability of the technique to examine the kinetics within individual lipoprotein species. Correction of enrichment data for proton exchange during combustion and reduction phases of sample preparation is an additional important procedural concern. In summary, the deuterated-water procedure is a useful tool in studies of human cholesterol synthesis that offers the advantages of short measurement interval, relative noninvasiveness, and provision of a direct index of synthesis in comparison with other available techniques.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Cardiology and Cardiovascular Medicine
Reference34 articles.
1. Regulation of cholesterol metabolism in man and in other species
2. Comparison of cholesterol turnover by sterol balance and input-output analysis, and a shortened way to estimate total exchangeable mass of cholesterol by the combination of the two methods;Samuel P;J Upid Res,1978
3. The interaction of cholesterol absorption and cholesterol synthesis in man;Grundy SM;J Upid Ra,1969
4. Turnover of plasma cholesterol in man
5. Cholesterol turnover in human plasma lipoproteins: studies with stable and radioactive isotopes
Cited by
69 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献