Correlation of Visco-Elastic Properties of Large Arteries with Microscopic Structure

Abstract

Nine aortas from recently killed dogs were sectioned into 21 or more ring segments supported horizontally on two hooks in Ringer's solution. One hook oscillated sinusoidally from .01 to 21 Hz to stretch the segments 1.2% in excess of 4 or more mean strain levels from 5 to 100%. The segments were kept at 4 temperature levels (0°, 20°, 37°, 60°C). The other hook was coupled to a force transducer. At frequencies below 1 Hz, the force registered was sinusoidal with the same frequency as the stretch which it led, unless the specimen contained demonstrably contracted smooth muscle; then the stress was nonlinear and lagged behind the strain at frequencies below .05 Hz. As frequencies rose above 1 Hz, the force amplitude rose to a maximum, resonating at frequency ω r , which was higher at higher initial strains. Concurrently the phase shift increased to 90° at another frequency ω 90 . When ω 90 = ω r , viscous losses were negligible, a fact confirmed by other data in this and other studies and generally implicating collagen. When ω 90 > ω r , viscous losses were appreciable, in agreement with other measurements and implicating either muscle or elastin. These two wall components could be distinguished from each other by responses to drugs and to changes in temperature. Absolute dynamic modulus, storage modulus, loss modulus, phase shift, and loss angle measured from stress-strain loops compared favorably with similar measurements published for other visco-elastic materials and with viscous and elastic constants obtained from stress-relaxation experiments on aorta.