Extracellular Vimentin/VWF (von Willebrand Factor) Interaction Contributes to VWF String Formation and Stroke Pathology

Author:

Fasipe Titilope A.12,Hong Sung-Ha23,Da Qi42,Valladolid Christian52,Lahey Matthew T.3,Richards Lisa M.6,Dunn Andrew K.6,Cruz Miguel A.42,Marrelli Sean P.23

Affiliation:

1. From the Section of Hematology-Oncology, Department of Pediatrics, Baylor College of Medicine Houston, TX (T.A.F.)

2. Center for Translational Research on Inflammatory Diseases, Michael E. DeBakey Veterans Affairs Medical Center, Houston, TX (T.A.F., S.-H.H., Q.D., C.V., M.A.C., S.P.M.)

3. Department of Neurology, McGovern Medical School at UTHealth, Houston, TX (S.-H.H., M.T.L., S.P.M.).

4. Department of Medicine, Baylor College of Medicine Houston, TX (Q.D., M.A.C.)

5. Department of Molecular Physiology and Biophysics, Baylor College of Medicine Houston, TX (C.V.)

6. Department of Biomedical Engineering, University of Texas at Austin (L.M.R., A.K.D.)

Abstract

Background and Purpose— VWF (von Willebrand factor) strings mediate spontaneous platelet adhesion in the vascular lumen, which may lead to microthrombi formation and contribute to stroke pathology. However, the mechanism of VWF string attachment at the endothelial surface is unknown. We tested the novel hypothesis that VWF strings are tethered to the endothelial surface through an interaction between extracellular vimentin and the A2 domain of VWF. We further explored the translational value of blocking this interaction in a model of ischemic stroke. Methods— Human endothelial cells and pressurized cerebral arteries were stimulated with histamine to elicit VWF string formation. Recombinant proteins and antibodies were used to block VWF string formation. Mice underwent transient middle cerebral artery occlusion with reperfusion. Just before recanalization, mice were given either vehicle or A2 protein (recombinant VWF A2 domain) to disrupt the vimentin/VWF interaction. Laser speckle contrast imaging was used to monitor cortical perfusion. Results— Pressurized cerebral arteries produced VWF strings following histamine stimulation, which were reduced in arteries from Vim KO (vimentin knockout) mice. VWF string formation was significantly reduced in endothelial cells incubated with A2 protein or antivimentin antibodies. Lastly, A2 protein treatment significantly improved cortical reperfusion after middle cerebral artery occlusion. Conclusions— We provide the first direct evidence of cerebral VWF strings and demonstrate that extracellular vimentin significantly contributes to VWF string formation via A2 domain binding. Lastly, we show that pharmacologically targeting the vimentin/VWF interaction through the A2 domain can promote improved reperfusion after ischemic stroke. Together, these studies demonstrate the critical role of VWF strings in stroke pathology and offer new therapeutic targets for treatment of ischemic stroke.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Advanced and Specialised Nursing,Cardiology and Cardiovascular Medicine,Clinical Neurology

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