Affiliation:
1. Department of Biology and Integrated Regenerative Research Institute San Diego State University San Diego CA
2. Department of Mathematics & Statistics San Diego State University San Diego CA
Abstract
Background
CardioChimeras produced by fusion of murine c‐kit
+
cardiac interstitial cells with mesenchymal stem cells promote superior structural and functional recovery in a mouse model of myocardial infarction compared with either precursor cell alone or in combination. Creation of human CardioChimeras (
hCCs
) represents the next step in translational development of this novel cell type, but new challenges arise when working with c‐kit
+
cardiac interstitial cells isolated and expanded from human heart tissue samples. The objective of the study was to establish a reliable cell fusion protocol for consistent optimized creation of
hCC
s and characterize fundamental
hCC
properties.
Methods and Results
Cell fusion was induced by incubating human c‐kit
+
cardiac interstitial cells and mesenchymal stem cells at a 2:1 ratio with inactivated Sendai virus. Hybrid cells were sorted into 96‐well microplates for clonal expansion to derive unique cloned
hCC
s, which were then characterized for various cellular and molecular properties.
hCC
s exhibited enhanced survival relative to the parent cells and promoted cardiomyocyte survival in response to serum deprivation in vitro.
Conclusions
The generation of
hCC
is demonstrated and validated in this study, representing the next step toward implementation of a novel cell product for therapeutic development. Feasibility of creating human hybrid cells prompts consideration of multiple possibilities to create novel chimeric cells derived from cells with desirable traits to promote healing in pathologically damaged myocardium.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Cardiology and Cardiovascular Medicine
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献