Affiliation:
1. Department of Pediatrics, Division of Cardiology Duke University School of Medicine Durham NC
2. Department of Cardiology The First Affiliated Hospital, College of Medicine, Zhejiang University Hangzhou China
3. Department of Molecular and Human Genetics Baylor College of Medicine and Baylor Genetics Laboratories Houston TX
4. Department of Cell Biology Duke University School of Medicine Durham NC
Abstract
Background
As utilization of clinical exome sequencing (ES) has expanded, criteria for evaluating the diagnostic weight of incidentally identified variants are critical to guide clinicians and researchers. This is particularly important in genes associated with dilated cardiomyopathy (DCM), which can cause heart failure and sudden death. We sought to compare the frequency and distribution of incidentally identified variants in DCM‐associated genes between a clinical referral cohort with those in control and known case cohorts to determine the likelihood of pathogenicity among those undergoing genetic testing for non‐DCM indications.
Methods and Results
A total of 39 rare, non‐
TTN
DCM‐associated genes were identified and evaluated from a clinical ES testing referral cohort (n=14 005, Baylor Genetic Laboratories) and compared with a DCM case cohort (n=9442) as well as a control cohort of population variants (n=141 456) derived from the gnomAD database. Variant frequencies in each cohort were compared. Signal‐to‐noise ratios were calculated comparing the DCM and ES cohort with the gnomAD cohort. The likely pathogenic/pathogenic variant yield in the DCM cohort (8.2%) was significantly higher than in the ES cohort (1.9%). Based on signal‐to‐noise and correlation analysis, incidental variants found in
FLNC
,
RBM20
,
MYH6
,
DSP
,
ABCC9
,
JPH2
, and
NEXN
had the greatest chance of being DCM‐associated.
Conclusions
The distribution of pathogenic variants between the ES cohort and the DCM case cohort was gene specific, and variants found in the ES cohort were similar to variants found in the control cohort. Incidentally identified variants in specific genes are more associated with DCM than others.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Cardiology and Cardiovascular Medicine
Cited by
3 articles.
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