Study of the parameters of absorption into the bloodstream and excretion of the drug Gamavit after a single administration to laboratory mini-pigs-Reference-Cited by-同舟云学术

Study of the parameters of absorption into the bloodstream and excretion of the drug Gamavit after a single administration to laboratory mini-pigs

Published:2021-09-03 Issue:3 Volume:2021 Page:34-42
ISSN:2500-4379
Container-title:Russian veterinary journal
language:en
Short-container-title:

Author:

Narovlyanskiy Aleksandr¹,Sanin Aleksandr¹,Smirnov Valeriy²,Savchenko Alla³,Ramenskaya Galina⁴,Sanina Valentina¹,Kozhevnikova Tat'yana⁵,Kozlov Vasiliy¹,Zubashev Igor'¹,Gerasimova Ekaterina¹,Pronin Aleksandr¹

Affiliation:

1. The Gamaleya Scientific Research Institute of Epidemiology and Microbiology

2. Institute of Immunology of the Federal Medical and Biological Agency (FMBA) of Russia

3. Scientific Center of Biomedical Technology of the Federal Medical and Biological Agency

4. Pharmacy Institute of I. M. Sechenov First Moscow State Medical University of the Ministry of Health of Russia

5. Gamaleya Scientific Research Institute of Epidemiology and Microbiolo

Abstract

A pharmacokinetic study of the absorption into the bloodstream, bioavailability and excretion of Gamavit from the body after intramuscular administration to laboratory mini-pigs was conducted. Quantitative determination was carried out by HPLC using a fluorimetric detector, for which Gamavit was labeled with Cy5 dye, which was then used for mini-pigs inoculation. The developed methods for determining Gamavit in the blood and feces were validated according to the following validation parameters: selectivity, calibration curve, accuracy, precision, limit of quantitative determination, sample transfer, and sample stability. The confirmed analytical range of the method for Gamavit detection in blood plasma and feces was 1.00…50.0 mcg/ml. Maximum concentration of Gamavit in the blood of mini-pigs after a single intramuscular injection was 30.97 mcg/ml and was reached on average 15 minutes after administration. 24 hours following administration, Gamavit was still detected in the blood in insignificant amounts. The average half-life of Gamavit in the blood is 8.64±3.50 hours. After administration at a dose of 0.1 ml/kg, the clearance of the drug is 1.27 l/kg * h, the excretion rate at an effective concentration of 30 mg/l is 38 mg/kg*h, and the maintenance dose when using the drug 1 time a day is 0.9…1.0 ml. The detection of the label in the feces of the studied animals indicates that one of the ways Gamavit removal is excretion with the help of bile acids, as well as partial excretion with feces.

Publisher

Publishing House Logos Press

Reference23 articles.

1. Анников, В.В. Эффективность Гамавита при проведении дегельминтизации котят и щенков / В.В. Анников, А.В. Красников, А.В. Санин // Ветеринарная клиника. – 2018. – № 5. – С. 24-25., Annikov, V.V. Effektivnost' Gamavita pri provedenii degel'mintizacii kotyat i schenkov / V.V. Annikov, A.V. Krasnikov, A.V. Sanin // Veterinarnaya klinika. – 2018. – № 5. – S. 24-25.

2. Анников, В.В. Эффективность гамавита при терапии боррелиоза у собак / В.В. Анников, Л.В. Анникова, А.В. Санин, А.Н. Наровлянский, А.В. Пронин, В.Ю. Санина, Т.Н. Кожевникова // Ветеринария Кубани. – 2018. – № 4 – С. 15-18., Annikov, V.V. Effektivnost' gamavita pri terapii borrelioza u sobak / V.V. Annikov, L.V. Annikova, A.V. Sanin, A.N. Narovlyanskiy, A.V. Pronin, V.Yu. Sanina, T.N. Kozhevnikova // Veterinariya Kubani. – 2018. – № 4 – S. 15-18.

3. Вишневская, Т.Я. Сравнительный анализ гематологических показателей крови при стрессе и его иммунокоррекция препаратом гамавит / Т.Я. Вишневская, Л.Л. Абрамова // В сб.: «Актуальные вопросы ветеринарной медицины», Материалы одиннадцатой Сибирской ветеринарной конференции, 2012 – С. 59-60., Vishnevskaya, T.Ya. Sravnitel'nyy analiz gematologicheskih pokazateley krovi pri stresse i ego immunokorrekciya preparatom gamavit / T.Ya. Vishnevskaya, L.L. Abramova // V sb.: «Aktual'nye voprosy veterinarnoy mediciny», Materialy odinnadcatoy Sibirskoy veterinarnoy konferencii, 2012 – S. 59-60.

4. Глаголева, Т.И. Ферроглюкин и гамавит в коррекции антиагрегационных свойств сосудов у новорожденных телят с дефицитом железа / Т.И. Глаголева, С.Ю. Завалишина, И.Н. Медведев // Успехи современного естествознания. – 2013. – № 5. – С. 17., Glagoleva, T.I. Ferroglyukin i gamavit v korrekcii antiagregacionnyh svoystv sosudov u novorozhdennyh telyat s deficitom zheleza / T.I. Glagoleva, S.Yu. Zavalishina, I.N. Medvedev // Uspehi sovremennogo estestvoznaniya. – 2013. – № 5. – S. 17.

5. Григорьева, Е.А. Воздействие препарата Гамавит на активность естественных киллерных клеток / Е.А. Григорьева, А.В. Пронин, А.В. Санин, А.Н. Наровлянский, Т.Ю. Тимофеева, В.Ю. Санина, Т.Н. Степанова, Е.В. Герасимова, А.М. Иванова, Т.Н. Кожевникова // Ветеринария Кубани. – 2016. – №4. – С. 27-28., Grigor'eva, E.A. Vozdeystvie preparata Gamavit na aktivnost' estestvennyh killernyh kletok / E.A. Grigor'eva, A.V. Pronin, A.V. Sanin, A.N. Narovlyanskiy, T.Yu. Timofeeva, V.Yu. Sanina, T.N. Stepanova, E.V. Gerasimova, A.M. Ivanova, T.N. Kozhevnikova // Veterinariya Kubani. – 2016. – №4. – S. 27-28.