BACTERICIDAL PROPERTIES OF COMPOSITES OF METHYLENE BLUE AND CETYLPYRIDINIUM CHLORIDE WITH GLAUCONITE OBTAINED BY ADSORPTION METHOD

Abstract

Recently, there has been an increased interest in aluminosilicates of natural origin, both in connection with the application in water and soil treatment systems, and as a matrix for the creation of biologically active composites. Glauconite is an aluminosilicate widely distributed in nature, which is promising for the development of effective enterosorbents and detoxification methods. New bactericidal composites based on glauconite from the Beloozersky deposit in the Saratov region are proposed. The method of scanning electron microscopy was used to study the morphological characteristics of individual glauconite and its composites. And the trace element composition was determined by energy dispersive X-ray spectroscopy. It was also established that the nitrogen adsorption-desorption isotherms for the studied glauconite sample are of type IV according to the classification of Deming and Teller (BET classification), and the sample is characterized by the presence of mesopores from 2 to 50 nm in size. The specific surface area of enriched glauconite by BET was 22.78 m2/g, and the total pore volume (P/Po = 0.98) - 0.044 cm3/g. Composites with methylene blue, cetyl priridinium chloride and their mixture were obtained on the basis of the enriched fraction of glauconite by sorption immobilization. The sorption capacity of glauconite in relation to the above biologically active substances is determined. An increase in the antibacterial effect of the obtained composites on standard strains of Staphylococcus aureus ATCC 6538 P and Escherichia coli ATCC 25922 relative to individual substances was revealed. Thus, the work shows that glauconite and composites based on it is a promising object for creating new types of enterosorbents for subsequent use in medicine and veterinary medicine.