Correlating nuclear morphology and external force with combined atomic force microscopy and light sheet imaging separates roles of chromatin and lamin A/C in nuclear mechanics-Reference-Cited by-同舟云学术

Correlating nuclear morphology and external force with combined atomic force microscopy and light sheet imaging separates roles of chromatin and lamin A/C in nuclear mechanics

Published:2020-07-21 Issue:16 Volume:31 Page:1788-1801
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Hobson Chad M.¹,Kern Megan¹,O’Brien E. Timothy¹,Stephens Andrew D.²,Falvo Michael R.¹,Superfine Richard³

Affiliation:

1. Department of Physics and Astronomy, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599

2. Biology Department, The University of Massachusetts at Amherst, Amherst, MA 01003, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599

3. Department of Applied Physical Sciences, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599

Abstract

Chromatin and lamin A/C separately resist strain in nuclear volume and surface area, respectively, during compression as studied with combined atomic force microscopy and light sheet imaging. Chromatin decompaction further alters curvature dynamics during indentation.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

Reference81 articles.

1. A Nondimensional Model Reveals Alterations in Nuclear Mechanics upon Hepatitis C Virus Replication

2. Mechanics and Buckling of Biopolymeric Shells and Cell Nuclei

3. Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies

4. Nuclear lamin functions and disease

5. Coordinated increase of nuclear tension and lamin-A with matrix stiffness outcompetes lamin-B receptor that favors soft tissue phenotypes

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