COMPARISON OF SOME BIOLOGICAL ACTIVITIES AND CATECHIN TANNIN CONTENTS OF TWO JUNIPERUS AND PRUNUS SPECIES

Abstract

Objective: Qualitative and quantitative determination of catechin in fruits of J. communis var. saxatilis, J. oxycedrus subsp. oxycedrus, P. spinosa, and P. cerasifera was analyzed by LC-MS/MS using standards. Antidiabetic, antimicrobial, and antioxidant activities of fruit and cone extracts of these plants were evaluated. Qualitative analysis of secondary metabolites was also done. Material and Method: Antimicrobial activity was done with MIC. ABTS·+ and DPPH• scavenging activies were used antioxidant activity. α-Amylase and α-glucosidase inhibition assays were done for antidiabetic activity. Result and Discussion: JCS (IC50 = 578 ± 20 μg/ml), JOO (IC50 = 3706 ± 52 μg/ml), and PS (IC50 = 313 ± 7 μg/ml) extracts were observed to show a higher activity than acarbose (IC50 = 4061 ± 146 μg/ml). J. communis var. saxatilis fruit extract was observed with MIC values between (312.5 -2500 µg/ml) against all microorganisms. JCS extract has the highest phenolic composition and JOO has the lowest phenolic composition (µg GAE/ mg extract). JCS extract indicated the highest antioxidant activity. It was determined that plant containing the highest catechin (1173.3±5.77 ng/ml) and epigallocatechin (208±7.21 ng/ml) was JCS. In this research, it can be said that JCS with the highest tannin content shows the highest effects.