Abstract
The genus <i>Babesia</i> includes parasites that can induce human and animal babesiosis, which are common in tropical and subtropical regions of the world. The gut microbiota has not been examined in hamsters infected by <i>Babesia duncani</i>. Red blood cells infected with <i>B. duncani</i> were injected into hamsters through intraperitoneal route. To evaluate the changes in gut microbiota, DNAs were extracted from small intestinal contents, acquired from hamsters during disease development. Then, the V4 region of the 16S rRNA gene of bacteria was sequenced using the Illumina sequencing platform. Gut microbiota alternation and composition were assessed according to the sequencing data, which were clustered with >97.0% sequence similarity to create amplicon sequence variants (ASVs). Bacteroidetes and Firmicutes were made up of the major components of the gut microbiota in all samples. The abundance of Bacteroidetes elevated after <i>B. duncani</i> infection than the <i>B. duncani</i>-free group, while Firmicutes and Desulfobacterota declined. Alpha diversity analysis demonstrated that the shown ASVs were substantially decreased in the highest parasitemia group than <i>B. duncani</i>-free and lower parasitemia groups. Potential biomarkers were discovered by Linear discriminant analysis Effect Size (LEfSe) analysis, which demonstrated that several bacterial families (including Muribaculaceae, Desulfovibrionaceae, Oscillospiraceae, Helicobacteraceae, Clostridia UGG014, Desulfovibrionaceae, and Lachnospiraceae) were potential biomarkers in <i>B. duncani</i>-infected hamsters. This research demonstrated that <i>B. duncani</i> infectious can modify the gut microbiota of hamsters.
Funder
Science Fund for Creative Research Groups of Gansu Province
Natural Science Foundation of Gansu Province
Gansu Natural Science Foundation
Science and Technology Development Guiding Program of Lanzhou
Lanzhou University Second Hospital
National Science Foundation of China
ASTIP
NBCIS
Leading Fund of Lanzhou Veterinary Research Institute
SKLVEB
Publisher
Korean Society for Parasitology
Subject
Infectious Diseases,Parasitology
Cited by
1 articles.
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