Affiliation:
1. Department of Life Sciences and National Institute for Biotechnology in the Negev, Ben-Gurion University, Beer-Sheva, Israel
2. Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania
Abstract
ABSTRACT
While previous studies have shown that microtubules (MTs) are essential for maintaining the highly biased axial growth of the
Drosophila
bristle, the mechanism for this process has remained vague. We report that the MT minus-end marker, Nod-KHC, accumulates at the bristle tip, suggesting that the MT network in the bristle is organized minus end out. Potential markers for studying the importance of properly polarized MTs to bristle axial growth are Ik2 and Spindle-F (Spn-F), since mutations in
spn-F
and
ik2
affect bristle development. We demonstrate that Spn-F and Ik2 are localized to the bristle tip and that mutations in
ik2
and
spn-F
affect bristle MT and actin organization. Specifically, mutation in
ik2
affects polarized bristle MT function. It was previously found that the
hook
mutant exhibited defects in bristle polarity and that
hook
is involved in endocytic trafficking. We found that Hook is localized at the bristle tip and that this localization is affected in
ik2
mutants, suggesting that the contribution of MTs within the bristle shaft is important for correct endocytic trafficking. Thus, our results show that MTs are organized in a polarized manner within the highly elongated bristle and that this organization is essential for biased bristle axial growth.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
32 articles.
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