Diagnosis of Gastric Syphilis by Direct Immunofluorescence Staining and Real-Time PCR Testing-Reference-Cited by-同舟云学术

Diagnosis of Gastric Syphilis by Direct Immunofluorescence Staining and Real-Time PCR Testing

Published:2006-09 Issue:9 Volume:44 Page:3452-3456
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Chen Cheng-Yen¹,Chi Kai-Hua¹,George Robert W.¹,Cox David L.¹,Srivastava Amitabh²,Rui Silva Mário³,Carneiro Fátima⁴,Lauwers Gregory Y.²,Ballard Ronald C.¹

Affiliation:

1. Division of STD Prevention, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia

2. Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts

3. Department of Pathology, University Hospital of Coimbra, Coimbra, Portugal

4. Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP) & Faculty of Medicine/H.S. João, Porto, Portugal

Abstract

ABSTRACT We report on a case of gastric syphilis in a patient with chronic dyspepsia. The diagnosis was established by serology and the demonstration of spirochetes in diffusely inflammed gastric mucosa by staining with a fluorescent monoclonal antibody specific for pathogenic treponemes and by the detection of specific treponemal DNA sequences by a real-time PCR.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00721-06

Reference10 articles.

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4. Inagaki, H., T. kawai, M. Miyata, S. Nagaya, H. Tateyama, and T. Eimoto. 1996. Gastric syphilis: polymerase chain reaction detection of treponemal DNA in pseudolymphomatous lesions. Hum. Pathol.27:761-765.

5. Specific immunofluorescent staining of pathogenic treponemes with a monoclonal antibody

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