Effect of swab type and storage temperature on the isolation of Chlamydia trachomatis from clinical specimens

Author:

Mahony J B,Chernesky M A

Abstract

We evaluated various swabs for the recovery of Chlamydia trachomatis and found that only 7 of 14 swabs tested resulted in a greater than 50% recovery of organisms. Storage of chlamydial specimens at 4 degrees C for more than 1 day resulted in a rapid loss of viability, whereas freezing of specimens at -70 degrees C resulted in only a marginal decrease in the isolation rate.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

1. Chernesky M. and J. Mahony. 1982. A tissue culture procedure for the isolation of chlamydiae from large numbers of clinical specimens p. 291-294. In P. A. Mardh and K. K. Holmes (ed.) Chlamydial infections: Proceedings 5th International Sympo-NOTES 867

2. sium Lund Sweden June 15-19 1982. Elsevier Biomedical

3. Press Amsterdam.

4. Incubation of swab materials with herpes simplex virus;Crane L. R.;J. Infect. Dis.,1980

5. Detection of chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation;Gordon F. B.;J. Infect. Dis.,1969

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