Characterization of a Low Molecular Weight Antigenic Protein from the Envelope of Influenza Virus

Abstract

Eckert, Edward A. (The University of Michigan, Ann Arbor). Characterization of a low molecular weight antigenic protein from the envelope of influenza virus. J. Bacteriol. 92: 1430–1434. 1966.—An antigenic protein from the lipid-extracted residue of influenza virus strain PR8 was solubilized with urea-dithiothreitol (DTT). The protein subunits had a sedimentation coefficient of 2 S in urea-DTT and reassociated to a 4 S state on dialysis. This form of the envelope protein did not agglutinate erythrocytes, but reacted with strain-specific antisera in the complement-fixation and blocking-antigen tests.