Author:
Sauvage Virginie,Ar Gouilh Meriadeg,Cheval Justine,Muth Erika,Pariente Kevin,Burguiere Ana,Caro Valérie,Manuguerra Jean-Claude,Eloit Marc
Abstract
During a study of the fecal microbiomes from two healthy piglets using high-throughput sequencing (HTS), we identified a viral genome containing an open reading frame encoding a predicted polyprotein of 2,133 amino acids. This novel viral genome displayed the typical organization of picornaviruses, containing three structural proteins (VP0, VP3, and VP1), followed by seven nonstructural proteins (2A, 2B, 2C, 3A, 3B, 3Cpro, and 3Dpol). Given its particular relationship withParechovirus, we propose to name it “Pasivirus” forParechosister clade virus, with “Swine pasivirus 1” (SPaV1) as the type species. Fecal samples collected at an industrial farm from healthy sows and piglets from the same herd (25 and 75, respectively) with ages ranging from 4 to 28 weeks were analyzed for the presence of SPaV1 by one-step reverse transcription (RT)-PCR targeting a 3D region of 151 bp. SPaV1 was detected in fecal samples from 51/75 healthy piglets (68% of the animals) and in none of the 25 fecal samples from healthy sows, indicating that SPaV1 circulates through enteric infection of healthy piglets. We propose that SPaV1 represents the first member of a novelPicornaviridaegenus related to parechoviruses.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference58 articles.
1. Protein is linked to the 5′ end of poliovirus RNA by a phosphodiester linkage to tyrosine;Ambros;J. Biol. Chem.,1978
2. BarbknechtM. 2009. Characterization of an unclassified virus and survey for its presence in Wisconsin bluegill populations. M.Sc. thesis. University of Wisconsin—La Crosse, La Crosse, WI.
3. Rapid full-length cloning of nonpolyadenylated RNA virus genomes;Beckett;Curr. Protoc. Microbiol.,2007
4. Cleavage site analysis in picornaviral polyproteins: discovering cellular targets by neural networks;Blom;Protein Sci.,1996
5. Evaluation of high-throughput sequencing for identifying known and unknown viruses in biological samples;Cheval;J. Clin. Microbiol.,2011
Cited by
42 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献