Quantifying Live Microbial Load in Human Saliva Samples over Time Reveals Stable Composition and Dynamic Load-Reference-Cited by-同舟云学术

Quantifying Live Microbial Load in Human Saliva Samples over Time Reveals Stable Composition and Dynamic Load

Published:2021-02-23 Issue:1 Volume:6 Page:
ISSN:2379-5077
Container-title:mSystems
language:en
Short-container-title:mSystems

Author:

Marotz Clarisse¹^ORCID,Morton James T.²,Navarro Perris¹,Coker Joanna¹,Belda-Ferre Pedro¹,Knight Rob¹³⁴⁵,Zengler Karsten¹³⁴^ORCID

Affiliation:

1. Department of Pediatrics, University of California, San Diego, La Jolla, California, USA

2. Center for Computational Biology, Flatiron Institute, Simons Foundation, New York, New York, USA

3. Center for Microbiome Innovation, University of California, San Diego, La Jolla, California, USA

4. Department of Bioengineering, University of California, San Diego, La Jolla, California, USA

5. Department of Computer Science & Engineering, University of California, San Diego, La Jolla, California, USA

Abstract

Human microbiomes are dynamic ecosystems often composed of hundreds of unique microbial taxa. To detect fluctuations over time in the human oral microbiome, we developed a novel workflow to quantify live microbial cells with flow cytometry in parallel with next-generation sequencing, and applied this method to over 150 unstimulated, timed saliva samples.

Funder

DOD | United States Army | RDECOM | Army Research Office

HHS | NIH | National Institute of Dental and Craniofacial Research

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSystems.01182-20

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