Epstein-Barr Virus Infection of Cell Lines Derived from Diffuse Large B-Cell Lymphomas Alters MicroRNA Loading of the Ago2 Complex-Reference-Cited by-同舟云学术

Epstein-Barr Virus Infection of Cell Lines Derived from Diffuse Large B-Cell Lymphomas Alters MicroRNA Loading of the Ago2 Complex

Published:2019-02 Issue:3 Volume:93 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Ayoubian Hiresh¹²,Ludwig Nicole²,Fehlmann Tobias³,Menegatti Jennifer¹,Gröger Laura²,Anastasiadou Eleni⁴,Trivedi Pankaj⁵,Keller Andreas²³,Meese Eckart²,Grässer Friedrich A.¹

Affiliation:

1. Department of Virology, Saarland University Medical School, Homburg, Germany

2. Department of Human Genetics and Center of Human and Molecular Biology, Saarland University Medical School, Homburg, Germany

3. Chair for Clinical Bioinformatics, Saarland University, Saarbrücken, Germany

4. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA

5. Department of Experimental Medicine, La Sapienza University, Rome, Italy

Abstract

Diffuse large B-cell lymphoma (DLBCL) is a highly aggressive tumor of lymphoid origin which is occasionally Epstein-Barr virus (EBV) positive. MicroRNAs are found in most multicellular organisms and even in viruses such as EBV. They regulate the synthesis of proteins by binding to their cognate mRNA. MicroRNAs are tethered to their target mRNAs by “Argonaute” proteins. Here we compared the overall miRNA content of the Ago2 complex by differential loading to the overall content of miRNAs in two DLBCL cell lines and their EBV-converted counterparts. In all cell lines, the Ago2 load was different from the overall expression of miRNAs. In addition, the loading of the Ago2 complex was changed upon infection with EBV. This indicates that the virus not only changes the overall content of miRNAs but also influences the expression of proteins by affecting the Ago complexes.

Funder

Deutsche Forschungsgemeinschaft

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01297-18

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