Carbon Flow in Mercury Biomethylation by Desulfovibrio desulfuricans

Author:

Berman Mitchell1,Chase Theodore1,Bartha Richard1

Affiliation:

1. Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, New Jersey 08903

Abstract

Radiocarbon incorporation from pyruvate and serine into monomethylmercury by Desulfovibrio desulfuricans was consistent with the proposal that the methyl group originates from C-3 of serine. Immunodiagnostic assays measured 4 to 35 μg of tetrahydrofolate and 58 to 161 ng of cobalamin or a closely related cobalt porphyrin per g of cell protein in D. desulfuricans. The light-reversible inhibition of mercury methylation by propyl iodide in D. desulfuricans indicates methyl transfer by a cobalt porphyrin.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference21 articles.

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2. Enzymatic synthesis of methionine: chemical alkylation of the enzyme-bound cobamide;Brot N.;J. Biol. Chem.,1965

3. Sulfate-reducing bacteria: principal methylators of mercury in anoxic estuarine sediment;Compeau G. C.;Appl. Environ. Microbiol.,1985

4. Effect of salinity on mercury-methylating activity of sulfate-reducing bacteria in estuarine sediments;Compeau G. C.;Appl. Environ. Microbiol.,1987

5. Hungate R. E. 1969. A roll-tube method for cultivation of strict anaerobes p. 117-132. In J. R. Norris and D. W. Ribbons (ed.) Methods in microbiology vol. 3. Academic Press Inc. New York.

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