Oxygen requirements of yeasts

Author:

Visser W1,Scheffers W A1,Batenburg-van der Vegte W H1,van Dijken J P1

Affiliation:

1. Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands.

Abstract

Type species of 75 yeast genera were examined for their ability to grow anaerobically in complex and mineral media. To define anaerobic conditions, we added a redox indicator, resazurin, to the media to determine low redox potentials. All strains tested were capable of fermenting glucose to ethanol in oxygen-limited shake-flask cultures, even those of species generally regarded as nonfermentative. However, only 23% of the yeast species tested grew under anaerobic conditions. A comparative study with a number of selected strains revealed that Saccharomyces cerevisiae stands out as a yeast capable of rapid growth at low redox potentials. Other yeasts, such as Torulaspora delbrueckii and Candida tropicalis, grew poorly mu max, 0.03 and 0.05 h-1, respectively) under anaerobic conditions in mineral medium supplemented with Tween 80 and ergosterol. The latter organisms grew rapidly under oxygen limitation and then displayed a high rate of alcoholic fermentation. It can be concluded that these yeasts have hitherto-unidentified oxygen requirements for growth.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference33 articles.

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4. Barnett J. A. R. W. Payne and D. Yarrow. 1983. A guide to identifying and classifying yeasts. Cambridge University Press Cambridge.

5. Influence of oxygen tension on the physiology of Saccharomyces cerevisiae in continuous culture. Antonie van Leeuwenhoek J;Brown C. M.;Microbiol. Serol.,1971

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