Affiliation:
1. Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands.
Abstract
Type species of 75 yeast genera were examined for their ability to grow anaerobically in complex and mineral media. To define anaerobic conditions, we added a redox indicator, resazurin, to the media to determine low redox potentials. All strains tested were capable of fermenting glucose to ethanol in oxygen-limited shake-flask cultures, even those of species generally regarded as nonfermentative. However, only 23% of the yeast species tested grew under anaerobic conditions. A comparative study with a number of selected strains revealed that Saccharomyces cerevisiae stands out as a yeast capable of rapid growth at low redox potentials. Other yeasts, such as Torulaspora delbrueckii and Candida tropicalis, grew poorly mu max, 0.03 and 0.05 h-1, respectively) under anaerobic conditions in mineral medium supplemented with Tween 80 and ergosterol. The latter organisms grew rapidly under oxygen limitation and then displayed a high rate of alcoholic fermentation. It can be concluded that these yeasts have hitherto-unidentified oxygen requirements for growth.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference33 articles.
1. Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium;Andreasen A. A.;J. Cell. Comp. Physiol.,1953
2. Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium;Andreasen A. A.;J. Cell. Comp. Physiol.,1954
3. Effects of oxygen and glucose levels on lipid composition of yeast Candida utilis grown in continuous culture;Babi T.;Biotechnol. Bioeng.,1969
4. Barnett J. A. R. W. Payne and D. Yarrow. 1983. A guide to identifying and classifying yeasts. Cambridge University Press Cambridge.
5. Influence of oxygen tension on the physiology of Saccharomyces cerevisiae in continuous culture. Antonie van Leeuwenhoek J;Brown C. M.;Microbiol. Serol.,1971
Cited by
221 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献