Author:
Rodríguez-Díaz Jesús,Carbajo Rodrigo J.,Pineda-Lucena Antonio,Monedero Vicente,Yebra María J.
Abstract
ABSTRACTAlfB and AlfC α-l-fucosidases fromLactobacillus caseiwere used in transglycosylation reactions, and they showed high efficiency in synthesizing fucosyldisaccharides. AlfB and AlfC activities exclusively produced fucosyl-α-1,3-N-acetylglucosamine and fucosyl-α-1,6-N-acetylglucosamine, respectively. The reaction kinetics showed that AlfB can convert 23%p-nitrophenyl-α-l-fucopyranoside into fucosyl-α-1,3-N-acetylglucosamine and AlfC at up to 56% into fucosyl-α-1,6-N-acetylglucosamine.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
42 articles.
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