The Movement Protein of Cowpea Mosaic Virus Binds GTP and Single-Stranded Nucleic Acid In Vitro-Reference-Cited by-同舟云学术

The Movement Protein of Cowpea Mosaic Virus Binds GTP and Single-Stranded Nucleic Acid In Vitro

Published:2004-02 Issue:3 Volume:78 Page:1591-1594
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Carvalho C. M.¹,Pouwels J.²,van Lent J. W. M.¹,Bisseling T.²,Goldbach R. W.¹,Wellink J.²

Affiliation:

1. Laboratory of Virology, Department of Plant Sciences, Wageningen University, 6709 PD Wageningen

2. Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University, 6703 HA Wageningen, The Netherlands

Abstract

ABSTRACT The movement protein (MP) of Cowpea mosaic virus forms tubules in plasmodesmata to enable the transport of mature virions. Here it is shown that the MP is capable of specifically binding riboguanosine triphosphate and that mutational analysis suggests that GTP binding plays a role in the targeted transport of the MP. Furthermore, the MP is capable of binding both single-stranded RNA and single-stranded DNA in a non-sequence-specific manner, and the GTP- and RNA-binding sites do not overlap.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.78.3.1591-1594.2004

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