Severe Soft Tissue Infection Caused by a Non-Beta-Hemolytic Streptococcus pyogenes Strain Harboring a Premature Stop Mutation in the sagC Gene-Reference-Cited by-同舟云学术

Severe Soft Tissue Infection Caused by a Non-Beta-Hemolytic Streptococcus pyogenes Strain Harboring a Premature Stop Mutation in the sagC Gene

Published:2013-06 Issue:6 Volume:51 Page:1962-1965
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Jantsch Jonathan¹,Gerlach Roman G.²,Ensser Armin³,Dahesh Samira⁴,Popp Isabel¹,Heeg Christiane⁵,Bleiziffer Oliver⁶,Merz Thomas⁷,Schulz Theresia¹,Horch Raymund E.⁵,Bogdan Christian¹,Nizet Victor⁴,van der Linden Mark⁵

Affiliation:

1. Mikrobiologisches Institut-Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

2. Nachwuchsgruppe 3, Robert Koch-Institut, Wernigerode, Germany

3. Institut für Klinische und Molekulare Virologie, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

4. Department of Pediatrics and Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, USA

5. Nationales Referenzzentrum für Streptokokken, Abteilung Medizinische Mikrobiologie, Universitätsklinikum Aachen, Aachen, Germany

6. Plastische- und Handchirurgische Klinik, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

7. Praxis Dr. Merz, Hemmhofen, Germany

Abstract

ABSTRACT We recovered a non-beta-hemolytic Streptococcus pyogenes strain from a severe soft tissue infection. In this isolate, we detected a premature stop codon within the sagC gene of the streptolysin S (SLS) biosynthetic operon. Reintroduction of full-length sagC gene on a plasmid vector restored the beta-hemolytic phenotype to our clinical isolate, indicating that the point mutation in sagC accounted for loss of hemolytic activity. To the best of our knowledge, this is the first report to demonstrate that a severe soft tissue infection can be caused by a non-beta-hemolytic S. pyogenes strain lacking a functional SagC.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00175-13

Reference23 articles.

1. Application of the polymerase chain reaction to study the M protein(-like) gene family in beta-hemolytic streptococci;Podbielski A;Med. Microbiol. Immunol,1991

2. A new closed-tube multiplex real-time PCR to detect eleven superantigens of Streptococcus pyogenes identifies a strain without superantigen activity;Lintges M;Int. J. Med. Microbiol,2007

3. Epidemiology of invasive Streptococcus pyogenes disease in Germany during 2003–2007;Imohl M;FEMS Immunol. Med. Microbiol,2010

4. Streptococcus pyogenes Isolates Causing Severe Infections in Norway in 2006 to 2007: emm Types, Multilocus Sequence Types, and Superantigen Profiles

5. Discovery of a widely distributed toxin biosynthetic gene cluster;Lee SW;Proc. Natl. Acad. Sci. U. S. A,2008

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