Optimization of Low-Biomass Sample Collection and Quantitative PCR-Based Titration Impact 16S rRNA Microbiome Resolution-Reference-Cited by-同舟云学术

Optimization of Low-Biomass Sample Collection and Quantitative PCR-Based Titration Impact 16S rRNA Microbiome Resolution

Published:2022-12-21 Issue:6 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Clokie Benjamin Gregory James¹^ORCID,Elsheshtawy Ahmed¹²,Albalat Amaya¹,Nylund Are³,Beveridge Allan¹,Payne Chris J.¹,MacKenzie Simon¹

Affiliation:

1. Institute of Aquaculture, University of Stirling, Stirling, United Kingdom

2. Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafr El Sheikh City, Egypt

3. Fish Disease Research Group, Department of Biological Sciences, University of Bergen, Bergen, Norway

Abstract

The interaction between the fish gill and surrounding bacteria-rich water provides an intriguing model for examining the interaction between the fish, free-floating bacteria, and the bacterial microbiome on the gill surface. Samples that are inherently low in bacteria, or that have components that inhibit the ability to produce libraries that identify the components of microbial communities, present significant challenges.

Funder

Fiskeri - og havbruksnæringens forskningsfond

Newton-Musharafa Fund

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02255-22

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2. Dynamic gill and mucus microbiomes during a gill disease episode in farmed Atlantic salmon