CRISPR-Cas13a-Based Assay for Accurate Detection of OXA-48 and GES Carbapenemases-Reference-Cited by-同舟云学术

CRISPR-Cas13a-Based Assay for Accurate Detection of OXA-48 and GES Carbapenemases

Published:2023-08-17 Issue:4 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Ortiz-Cartagena Concha¹²,Pablo-Marcos Daniel³,Fernández-García Laura¹²,Blasco Lucía¹²^ORCID,Pacios Olga¹²,Bleriot Inés¹²,Siller María³,López María¹²,Fernández Javier⁴⁵^ORCID,Aracil Belén⁶⁷,Fraile-Ribot Pablo Arturo⁸⁷,García-Fernández Sergio³,Fernández-Cuenca Felipe⁹,Hernández-García Marta¹⁰⁷^ORCID,Cantón Rafael¹⁰⁷,Calvo-Montes Jorge³⁷,Tomás María¹²^ORCID

Affiliation:

1. Multidisciplinary and Translational Microbiology Group (MicroTM), Biomedical Research Institute of A Coruña (INIBIC), Microbiology Service, University Hospital of A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain

2. Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA) on behalf of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain

3. Microbiology Service, University Hospital Marqués de Valdecilla – IDIVAL, Santander, Spain

4. Microbiology Service, University Hospital Central de Asturias. Translational Microbiology Group, ISPA, Oviedo, Spain

5. CIBER de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain

6. Reference and Research Laboratory for Antibiotic Resistance and Health Care Infections, National Centre for Microbiology, Institute of Health Carlos III, Majadahonda, Madrid, Spain

7. CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain

8. Microbiology Service, University Hospital Son Espases and Health Research Institute Illes Balears (IdISBa), Palma de Mallorca, Spain

9. Clinical Unit of Infectious Diseases and Microbiology, University Hospital Virgen Macarena, Institute of Biomedicine of Sevilla (University Hospital Virgen Macarena/CSIC/University of Sevilla), Sevilla, Spain

10. Microbiology Service, University Hospital Ramón y Cajal and Ramón y Cajal Health Research Institute (IRYCIS), Madrid, Spain

Abstract

Carbapenems are one of the last-resort antibiotics for defense against multidrug-resistant pathogens. Multiple nucleic acid amplification methods, including multiplex PCR, multiplex loop-mediated isothermal amplification (LAMP) and multiplex RPAs, can achieve rapid, accurate, and simultaneous detection of several resistance genes to carbapenems in a single reaction.

Funder

MEC | Instituto de Salud Carlos III

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01329-23

Reference31 articles.

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Carbapenemase Producing Gram-Negative Bacteria in Tunisia: History of Thirteen Years of Challenge

5. The Global Ascendency of OXA-48-Type Carbapenemases

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