Laboratory-Generated DNA Can Cause Anomalous Pathogen Diagnostic Test Results

Author:

Robinson-McCarthy Lindsey R.1,Mijalis Alexander J.1,Filsinger Gabriel T.2,de Puig Helena34,Donghia Nina M.3,Schaus Thomas E.3,Rasmussen Robert A.3,Ferreira Raphael13,Lunshof Jeantine E.356,Chao George1,Ter-Ovanesyan Dmitry3,Dodd Oliver13,Kuru Erkin13,Sesay Adama M.3,Rainbow Joshua37,Pawlowski Andrew C.13,Wannier Timothy M.1,Angenent-Mari Nicolaas M.348,Najjar Devora349,Yin Peng32,Ingber Donald E.31011ORCID,Tam Jenny M.13ORCID,Church George M.131213

Affiliation:

1. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA

2. Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA

3. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA

4. Institute for Medical Engineering and Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA

5. Department of Global Health and Social Medicine, Harvard Center for Bioethics, Harvard Medical School, Boston, Massachusetts, USA

6. European Research Institute for the Biology of Ageing, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

7. Department of Electrical and Electronic Engineering, University of Bath, Bath, United Kingdom

8. Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA

9. MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA

10. Vascular Biology Program, Department of Surgery, Boston Children’s Hospital and Harvard Medical School, Boston, Massachusetts, USA

11. Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA

12. Harvard-MIT Program in Health Sciences and Technology, Cambridge, Massachusetts, USA

13. Blavatnik Institute, Harvard Medical School, Boston, Massachusetts, USA

Abstract

To meet the challenges imposed by the COVID-19 pandemic, research laboratories shifted their focus and clinical diagnostic laboratories developed and utilized new assays. Nucleic acid-based testing became widespread and, for the first time, was used as a prophylactic measure.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Reference22 articles.

1. Diagnostics for SARS-CoV-2 infections

2. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets

3. Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories

4. Johns Hopkins University Coronavirus Resource Center. 2021. Daily state-by-state testing trends. Johns Hopkins University Baltimore MD. https://coronavirus.jhu.edu/testing/individual-states. Accessed June 28 2021.

5. Pierce H McKinney R Jr. 2020. AAMC recommendations for COVID-19 testing: the current state and the way forward. Association of American Medical Colleges Washington DC. https://www.aamc.org/covidroadmap/testing.

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