Affiliation:
1. Department of Environmental & Energy Engineering, Yonsei University , Wonju, South Korea
2. Bio-Chemical Analysis Group, Centre for Research Equipment, Korea Basic Science Institute , Cheongju, South Korea
3. Department of Systems Biotechnology, Chung-Ang University , Anseong, South Korea
Abstract
ABSTRACT
Microbial interactions are determined by competition, cooperation, and inaction, depending on the transcriptional response to the metabolites secreted by each other. The consequence of these interactions is reflected in the cell phenotype by the composition of cell materials, including lipids, nucleotides, and proteins. We studied the phenotypic plasticity of single cells involved in microbial interactions using Raman spectroscopy and evaluated the types of interactions by linking phenotypes to transcriptional profiles. We used a membrane-based co-culture system to induce chemical interactions between skin-dominant bacteria and fungi,
Staphylococcus
and
Malassezia. Staphylococcus
species exhibited an amensalism interaction with
Malassezia
based on growth and cell viability.
Malassezia
co-cultured with
S. epidermidis
and
S. aureus
resulted in significant changes in the Raman spectra of nucleic acids and proteins in the Raman phenotypes, respectively. The observed differences in phenotypes during co-culture with
Malassezia
were significantly associated with changes in transcriptomic profiles, which demonstrated variations in the defense/resistance and biofilm-formation mechanisms of
S. epidermidis,
whereas no meaningful changes were observed for the transcriptome of
S. aureus
. These approaches provide a robust, simple, and reproducible method for comprehending bacterial–fungal interactions.
IMPORTANCE
Evaluating bacterial–fungal interactions is important for understanding ecological functions in a natural habitat. Many studies have defined bacterial–fungal interactions according to changes in growth rates when co-cultivated. However, the current literature lacks detailed studies on phenotypic changes in single cells associated with transcriptomic profiles to understand the bacterial-fungal interactions. In our study, we measured the single-cell phenotypes of bacteria co-cultivated with fungi using Raman spectroscopy with its transcriptomic profiles and determined the consequence of these interactions in detail. This rapid and reliable phenotyping approach has the potential to provide new insights regarding bacterial–fungal interactions.
Funder
National Research Foundation of Korea
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology
Cited by
1 articles.
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