Comprehensive Comparison of Seven SARS-CoV-2-Specific Surrogate Virus Neutralization and Anti-Spike IgG Antibody Assays Using a Live-Virus Neutralization Assay as a Reference-Reference-Cited by-同舟云学术

Comprehensive Comparison of Seven SARS-CoV-2-Specific Surrogate Virus Neutralization and Anti-Spike IgG Antibody Assays Using a Live-Virus Neutralization Assay as a Reference

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Graninger Marianne¹,Jani Claudia Maria¹,Reuberger Elisabeth¹,Prüger Katja¹,Gaspar Philipp¹,Springer David Niklas¹,Borsodi Christian¹,Weidner Lisa²,Rabady Susanne³,Puchhammer-Stöckl Elisabeth¹^ORCID,Jungbauer Christof²,Höltl Eva⁴,Aberle Judith Helene¹,Stiasny Karin¹^ORCID,Weseslindtner Lukas¹^ORCID

Affiliation:

1. Center for Virology, Medical University of Vienna, Vienna, Austria

2. Austrian Red Cross, Blood Service for Vienna, Lower Austria, and Burgenland, Vienna, Austria

3. Karl Landsteiner University of Health Sciences, Department of General Health Studies, Division General and Family Medicine, Krems, Austria

4. Center for Public Health, Medical University of Vienna, Vienna, Austria

Abstract

While the measurement of neutralizing antibodies is considered a valuable tool in assessing protection against SARS-CoV-2, neutralization tests employ live-virus isolates and cell culture, requiring advanced laboratory biosafety levels. Including a large sample panel (over 700 samples), this study provides adapted cutoff values calculated for seven commercial immunoassays (including four surrogate neutralization assays and a protein-based microarray) that robustly correlate with specific titers of neutralizing antibodies.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02314-22

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