Affiliation:
1. Department of Pathology and Laboratory Medicine, Emory University School of Medicine
2. Clinical Microbiology Laboratory, Emory University Hospital
3. Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
Abstract
ABSTRACT
We compared a rapid slide latex agglutination test (LAT; Oxoid, Basingstoke, United Kingdom) that detects penicillin binding protein 2a (PBP2a) with MicroScan conventional panels (Dade Behring, West Sacramento, CA) for detection of oxacillin resistance in
Staphylococcus aureus
. The PBP2a LAT demonstrated 99% agreement with MicroScan oxacillin MIC results for 388 isolates of
S. aureus
. All 249 oxacillin-resistant isolates gave strong positive reactions in the LAT (100% sensitivity). Three of the 139 oxacillin-susceptible isolates were also strongly positive and one was weakly positive in the LAT (97.1% specificity). The three oxacillin-susceptible isolates with strongly positive reactions were further characterized. The
mecA
gene was detected in all three by PCR; one isolate was determined to be resistant to oxacillin by reference broth microdilution testing (MIC, 8 μg/ml), one isolate was inducibly resistant to oxacillin (MIC of 16 μg/ml after overnight induction), and one isolate remained susceptible regardless of the method used for testing. Sequence analysis of a 2.1-kb gene fragment of the
mecA
gene from the susceptible isolate revealed a one-base substitution at nucleotide position 1449 which results in a Met-to-Ile change for amino acid residue 483. This amino acid substitution has not been previously reported and may be associated with a change in the function of PBP2a resulting in oxacillin susceptibility. An additional 487 isolates were tested in parallel with the both the LAT and MicroScan panels using criteria in which only strong (3 to 4+) or repeatedly weak (1 to 2+) LAT reactions were considered positive, and the results showed 99.4% agreement. The PBP2a LAT provided rapid and reliable detection of oxacillin resistance and proved a useful adjunct to the phenotypic method. Both methods provided reliable detection of oxacillin-resistant
S. aureus
and facilitated the discovery of a novel, functionally impaired form of PBP2a.
Publisher
American Society for Microbiology
Cited by
24 articles.
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