CULTURE AND FLUORESCENT-ANTIBODY METHODS IN DIAGNOSIS OF WHOOPING COUGH

Author:

Holwerda Jack1,Eldering Grace1

Affiliation:

1. Michigan Department of Health Laboratories, Grand Rapids, Michigan

Abstract

Holwerda, Jack (Michigan Department of Health, Grand Rapids) and Grace Eldering . Culture and fluorescent-antibody methods in diagnosis of whooping cough. J. Bacteriol. 86: 449–451. 1963.—Nasopharyngeal swabs from 517 suspected whooping-cough patients were examined by culture and by fluorescent-antibody (FA) staining procedures applied to direct slide preparations. A total of 138 were positive by both methods, 25 by culture only, and 25 by FA only. The FA technique was also used in the identification of young cultures. It was shown that a positive culture report could be speeded up by about 1 day by this method. Without FA, onehalf the positive culture reports were made in 3 days; with FA, 75% were reported in a similar period.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference6 articles.

1. Nasopharyngeal cultures in pertussis;BRADFORD W. L.;Proc. Soc. Exptl. Biol. Med.,1940

2. Diagnosis of pertussis by fluorescent antibody staining of nasopharyngeal smears;DONALDSON P.;Am. J. Diseases Children,1960

3. Fluorescent antibody staining and agglutination reactions in Bordetella pertussis cultures;ELDERING G., W.;J. Bacteriol.,1962

4. Flourescent antibody techniques;KENDRICK P. L.;Am. J. Diseases Children,1961

5. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent antibody technique with modification of its application;MARSHALL J. D.;Proc. Soc. Exptl. Biol. Med.,1958

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