Affiliation:
1. Departments of Animal Diseases and Animal Industries, University of Connecticut, Storrs, Connecticut
2. Biophysics Department, Yale University, New Haven, Connecticut
Abstract
Tourtellotte, Mark
E. (University of Connecticut, Storrs),
Robert G. Jensen, George W. Gander, and Harold J. Morowitz
. Lipid composition and synthesis in the pleuropneumonia-like organism
Mycoplasma gallisepticum
. J. Bacteriol.
86:
370–379. 1963.—A simple method of extracting lipids from cells of
Mycoplasma gallisepticum
by use of silicic acid columns is described. Proteolipids (peptides) extracted with chloroform-methanol (2:1) by other methods were not extracted with the lipid by this method, nor were proteins and nucleic acids. Fractionation of lipids of
M. gallisepticum
demonstrated the presence of saturated hydrocarbons, free fatty acids, cholesterol, cholesterol esters, di- and triglycerides, phosphatidic acids, cephalins, inositides, phosphatidyl choline, and sphingomyelin. The fatty acid composition of the various fractions was also determined. The positive identification of cholesterol and cholesterol esters in this organism by chromatography, melting point, and infrared spectroscopy confirms reports by others that cholesterol is present in the pleuropneumonia group of microorganisms. The incorporation of P
32
orthophosphate into four phospholipid fractions, of oleic acid-
1-C
14
into neutral and phospholipids, and cholesterol-
4-C
14
into cholesterol esters clearly demonstrated the ability of
M. gallisepticum
to synthesize these lipids from simpler compounds. Between 70 and 80% of the lipid of this organism was found in the membrane.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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