A Practical Guide to Small Protein Discovery and Characterization Using Mass Spectrometry-Reference-Cited by-同舟云学术

A Practical Guide to Small Protein Discovery and Characterization Using Mass Spectrometry

Published:2022-01-18 Issue:1 Volume:204 Page:
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Ahrens Christian H.¹^ORCID,Wade Joseph T.²³^ORCID,Champion Matthew M.⁴^ORCID,Langer Julian D.⁵⁶^ORCID

Affiliation:

1. Agroscope, Method Development and Analytics & SIB Swiss Institute of Bioinformatics, Wädenswil, Switzerland

2. Wadsworth Center, New York State Department of Health, Albany, New York, USA

3. Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York, USA

4. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA

5. Mass Spectrometry and Proteomics, Max Planck Institute of Biophysics, Frankfurt am Main, Germany

6. Proteomics, Max Planck Institute for Brain Research, Frankfurt am Main, Germany

Abstract

Small proteins of up to ∼50 amino acids play important physiological roles across all domains of life. Mass spectrometry is an ideal approach to detect and characterize small proteins, but many aspects of standard mass spectrometry workflows are biased against small proteins due to their size. Here, we highlight applications of mass spectrometry to study small proteins, emphasizing modifications to standard workflows to optimize the detection of small proteins.

Funder

Swiss National Science Foundation

HHS | NIH | National Institute of General Medical Sciences

Deutsche Forschungsgemeinschaft

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.00353-21

Reference142 articles.

1. Large-Scale Analyses of Human Microbiomes Reveal Thousands of Small, Novel Genes