Molecular Cloning and Characterization of Fengycin Synthetase Gene fenB from Bacillus subtilis-Reference-Cited by-同舟云学术

Molecular Cloning and Characterization of Fengycin Synthetase Gene fenB from Bacillus subtilis

Published:1998-03 Issue:5 Volume:180 Page:1338-1341
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Lin Guang-Huey¹²,Chen Chyi-Liang²,Tschen Johannes Scheng-Ming³,Tsay San-San¹,Chang Yu-Sun²,Liu Shih-Tung²

Affiliation:

1. Graduate Institute of Botany, National Taiwan University, Taipei, 106,1

2. Molecular Genetics Laboratory, Department of Microbiology and Immunology, Chang-Gung University, Kwei-Shan, Taoyuan, 333,2 Taiwan

3. Graduate Institute of Botany, National Chung-Hsing University, Taichung, 402,3 and

Abstract

ABSTRACT A fengycin synthetase gene, fenB , has been cloned and sequenced. The protein (FenB) encoded by this gene has a predicted molecular mass of 143.6 kDa. This protein was overexpressed in Escherichia coli and was purified to near homogeneity by affinity chromatography. Experimental results indicated that the recombinant FenB has a substrate specificity toward isoleucine with an optimum temperature of 25°C, an optimum pH of 4.5, a K m value of 922 μM, and a turnover number of 236 s −1 . FenB also consists of a thioesterase domain, suggesting that this protein may be involved in the activation of the last amino acid of fengycin.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.180.5.1338-1341.1998

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