Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay-Reference-Cited by-同舟云学术

Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay

Published:1996-08 Issue:8 Volume:34 Page:2030-2032
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hassan-King M¹,Baldeh I¹,Adegbola R¹,Omosigho C¹,Usen S O¹,Oparaugo A¹,Greenwood B M¹

Affiliation:

1. Medical Research Council Laboratories, Fajara, The Gambia, West Africa.

Abstract

A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptococcus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.34.8.2030-2032.1996

Reference8 articles.

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