Affiliation:
1. Department of Medicine, Stanford University, Stanford, California 94305
Abstract
A method for preparing large membrane fragments and cell ghosts was developed for uninfected and Rous sarcoma virus-transformed chicken embryo fibroblasts in culture. Membrane proteins were analyzed by electrophoresis in acrylamide gels containing sodium dodecyl sulfate. A major amino-acid-containing component of uninfected cell membranes was greatly diminished in amount or absent in membranes of virus-transformed cells. This component, called MP-1, had an electrophoretic mobility in sodium dodecyl sulfate-containing gels similar to that of a protein of a mol wt of 1.42 × 10
5
. MP-1 was not altered by changes in cell growth rate or in cells infected with the nontransforming virus RAV-1.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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