A Comparative Study of Methods To Validate Formaldehyde Decontamination of Biological Safety Cabinets

Author:

Munro Kerry1,Lanser Janice1,Flower Robert23

Affiliation:

1. Infectious Diseases Laboratories, Institute of Medical and Veterinary Science,1 and

2. School of Pharmacy and Medical Sciences, University of South Australia,2 Adelaide, South Australia, and

3. Pacific Laboratory Medicine Services, Royal North Shore Hospital, St. Leonards, New South Wales,3Australia

Abstract

ABSTRACT Methods of validation of formaldehyde decontamination of biological safety cabinets were compared. Decontamination of metal strips inoculated with Mycobacterium bovis , poliovirus, or Bacillus spp. spores was compared with the results obtained with three biological indicators. Conditions for successful decontamination, particularly relative humidity, were defined. The Attest 1291 biological indicator was the only biological indicator which was an aid in the detection of gross decontamination failure.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference13 articles.

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3. Problems in the disinfection of class 1 microbiological safety cabinets.;Everall P. H.;J. Clin. Pathol.,1982

4. Effect of Relative Humidity on the Penetrability and Sporicidal Activity of Formaldehyde

5. A study of conventional formaldehyde fumigation methods.;Lach V. H.;J. Appl. Bacteriol.,1990

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