Affiliation:
1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Abstract
A mini-Tn10-kan insertion mutation identified a gene in the chromosome of Escherichia coli required for colicin V production from plasmid pColV-K30. With the complete restriction map of E. coli, the mutation was rapidly mapped to 50.0 min, within the purF operon. Sequence analysis showed that the insertion occurred in a gene with no previously known function which is located directly upstream of purF. We designated this gene cvpA for colicin V production. The mutant requires adenine for growth, probably because of a polar effect on purF expression. However, an adenine auxotroph showed no defect in colicin V production, suggesting that the cvpA mutation is responsible for the effect on colicin V production. Two possible models of cvpA1 allele function are discussed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference27 articles.
1. Ausubel F. M. R. Brent R. Kingston D. Moore J. Seidman J. A. Smith and K. Struhl. 1987. Current protocols in molecular biology. John Wiley & Sons Inc. New York.
2. Bachmann B. J. 1987. Derivations and genotypes of some mutant derivatives of Escherichia coli K-12 p. 1190-1219. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology vol. 2. American Society for Microbiology Washington D.C.
3. Rapid and precise mapping of the Escherichia coli release factor genes by two physical approaches;Cheng C. L.;J. Bacteriol.,1988
4. An E. coli promoter induced by the cessation of growth;Connell N.;Mol. Microbiol.,1987
5. Cloning of immunity and structural genes for colicin V;Frick K. K.;J. Bacteriol.,1981
Cited by
45 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献