Comparing Immunoassays for SARS-CoV-2 Antibody Detection in Patients with and without Laboratory-Confirmed SARS-CoV-2 Infection-Reference-Cited by-同舟云学术

Comparing Immunoassays for SARS-CoV-2 Antibody Detection in Patients with and without Laboratory-Confirmed SARS-CoV-2 Infection

Published:2021-11-18 Issue:12 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Leuzinger Karoline¹²,Osthoff Michael³,Dräger Sarah³,Pargger Hans⁴,Siegemund Martin⁴,Bassetti Stefano³,Bingisser Roland⁵,Nickel Christian H.⁵,Tschudin-Sutter Sarah⁶,Khanna Nina⁶,Rentsch Katharina⁷,Battegay Manuel⁶,Egli Adrian⁸⁹,Hirsch Hans H.¹²⁶^ORCID

Affiliation:

1. Clinical Virology, University Hospital Basel, Basel, Switzerland

2. Transplantation & Clinical Virology, Department of Biomedicine, University of Basel, Basel, Switzerland

3. Internal Medicine, University Hospital Basel, Basel, Switzerland

4. Intensive Care Unit, University Hospital Basel, Basel, Switzerland

5. Emergency Medicine, University Hospital Basel, Basel, Switzerland

6. Infectious Diseases & Hospital Epidemiology, University Hospital Basel

7. Clinical Chemistry, Laboratory Medicine, University Hospital Basel, Basel, Switzerland

8. Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland

9. Clinical Bacteriology and Mycology, University Hospital Basel, Basel, Switzerland

Abstract

Commercially available SARS-CoV-2-directed antibody assays may assist in diagnosing past exposure to SARS-CoV-2 antigens. We cross-compared the following eight immunoassays detecting antibodies against SARS-CoV-2 nucleocapsid (N) or spike (S) antigens in three cohorts consisting of 859 samples from 622 patients: (i) EDI novel coronavirus COVID-19 (Epitope), (ii) RecomWell SARS-CoV-2 (Mikrogen), (iii) COVID-19 ELISA (VirCell), (iv) Elecsys anti-SARS-CoV-2 N (Roche), (v) Liaison SARS-CoV-2 S1/S2 (DiaSorin), (vi) anti-SARS-CoV-2 ELISA (EuroImmun), (vii) Elecsys anti-SARS-CoV-2 S (Roche), and (viii) Liaison SARS-CoV-2 TrimericS (DiaSorin).

Funder

Universität Basel

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01381-21

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