Mesophilic Fermentation of Renewable Biomass: Does Hydraulic Retention Time Regulate Methanogen Diversity?

Author:

Krakat Niclas12,Schmidt Stefan3,Scherer Paul1

Affiliation:

1. Hamburg University of Applied Sciences, Research Centre of Lifetec Process Engineering, Lohbrügger Kirchstr. 65, 21033 Hamburg-Bergedorf, Germany

2. University of Hamburg, Department of Chemistry, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany

3. University of KwaZulu-Natal, Discipline of Microbiology, School of Biochemistry, Genetics and Microbiology, Pietermaritzburg 3201, South Africa

Abstract

ABSTRACT The present long-term study (about 1,100 days) monitored the diversity of methanogens during the mesophilic, anaerobic digestion of beet silage. Six fermentor samples were analyzed by ribosomal RNA gene restriction analysis, fluorescence in situ hybridization, and fluorescence microscopy. Hydrogenotrophic methanogens dominated within the population in all samples analyzed. Multidimensional scaling revealed that a rapid decrease in hydraulic retention time resulted in increased species richness, which in turn led to slightly higher CH 4 yields.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference49 articles.

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