Affiliation:
1. Department of Microbiology, Naval Medical Research Institute, Bethesda, Maryland 20014
Abstract
We compared the polynucleotide sequence relationships of three strains of
Chlamydia trachomatis
of human origin (MRC-1/G, TW-3, and Lgv), one of murine origin (MoPn), and the MN strain of
C. psittaci.
The four strains of
C. trachomatis
have the same base ratio, about 42.5 moles per cent guanine plus cytosine, which is significantly higher than the base ratio of MN (39.5). Single strands of deoxyribonucleic acid (DNA) fragments of MRC-1/G reassociated with immobilized DNA of TW-3 and Lgv almost as well as with the homologous DNA. The duplexes produced in these reactions were about equally thermostable. On the other hand, reassociations between MRC-1/G and MoPn involved 60 or 30% of the DNA, depending on the stringency of the conditions for reassociation, and the duplexes were thermolabile. MoPn reassociated only to a very small degree with MN. We also compared glucose catabolism of MRC-1/G, MoPn, and MN under several sets of conditions. These tests failed to reveal any qualitative phenotypic differences among the three strains. It can be concluded that, judging by polynucleotide sequence, the three human strains of
C. trachomatis
are closely related but appreciably different from a murine strain.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
43 articles.
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